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ELUCIDATION OF THE TFM METABOLISMS IN SEA LAMPREY AND NON-TARGET FISH
Belinda Huerta 2, Yu-Wen Chung-Davidson2, Ugo Bussy2, Ke Li2, Weiming Li2
2Department of Fisheries & Wildlife, Michigan State University, East Lansing, MI 48824
TFM metabolism in vivo was studied in sea lamprey and non-target species, which allowed the description and synthesis of several novel metabolites. Accordingly, a quantitative method was developed for the assessment of TFM and its metabolites in different matrices, including fish liver tissue and water samples. Among the novel metabolites described, the presence of nitro reductive metabolites as a consequence of the reduction of TFM was hypothesized to be a determining factor for sea lamprey sensitivity to this lampricide. Mitochondrial oxidative phosphorylation in sea lamprey was studied when exposed to both TFM and amino TFM, as well as their non-fluorinated counterparts in order to determine if reductive metabolites could also uncouple the ATP synthase or affect other complexes in the electron transfer chain to the same degree of TFM. Amino TFM was found to affect mitochondrial respiration, although at a higher concentration than TFM, which suggests a lower toxicological effect than the parent compound.